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Flow cytometric analysis of <t>CD1a</t> and CD83 expression on the surface of DCs at different time points. (A) Expression of CDla on Day 1 of Culture; (B) Expression of CDla on Day 8 of Culture; (C) Statistical Analysis Results of CDla on Day 1 and Day 8 of Culture; (D) Expression of CD83 on Day 1 of Culture; (E) Expression of CD83 on Day 8 of Culture; (F) Statistical Analysis Results of CD83 on Day 1 and Day 8 of Culture. It is evident that the maturation rate of DCs induced by GM-CSF and IL-4 has been significantly enhanced, thereby facilitating sample preparation for subsequent experiments.Statistical significance is indicated in the figure (*p < 0.05, **P < 0.01, ***P < 0.001).
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Flow cytometric analysis of <t>CD1a</t> and CD83 expression on the surface of DCs at different time points. (A) Expression of CDla on Day 1 of Culture; (B) Expression of CDla on Day 8 of Culture; (C) Statistical Analysis Results of CDla on Day 1 and Day 8 of Culture; (D) Expression of CD83 on Day 1 of Culture; (E) Expression of CD83 on Day 8 of Culture; (F) Statistical Analysis Results of CD83 on Day 1 and Day 8 of Culture. It is evident that the maturation rate of DCs induced by GM-CSF and IL-4 has been significantly enhanced, thereby facilitating sample preparation for subsequent experiments.Statistical significance is indicated in the figure (*p < 0.05, **P < 0.01, ***P < 0.001).
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Flow cytometric analysis of CD1a and CD83 expression on the surface of DCs at different time points. (A) Expression of CDla on Day 1 of Culture; (B) Expression of CDla on Day 8 of Culture; (C) Statistical Analysis Results of CDla on Day 1 and Day 8 of Culture; (D) Expression of CD83 on Day 1 of Culture; (E) Expression of CD83 on Day 8 of Culture; (F) Statistical Analysis Results of CD83 on Day 1 and Day 8 of Culture. It is evident that the maturation rate of DCs induced by GM-CSF and IL-4 has been significantly enhanced, thereby facilitating sample preparation for subsequent experiments.Statistical significance is indicated in the figure (*p < 0.05, **P < 0.01, ***P < 0.001).

Journal: Frontiers in Molecular Biosciences

Article Title: Deciphering the circRNA-Mediated ceRNA regulatory network in dendritic cells during H37Ra and BCG infection

doi: 10.3389/fmolb.2026.1764518

Figure Lengend Snippet: Flow cytometric analysis of CD1a and CD83 expression on the surface of DCs at different time points. (A) Expression of CDla on Day 1 of Culture; (B) Expression of CDla on Day 8 of Culture; (C) Statistical Analysis Results of CDla on Day 1 and Day 8 of Culture; (D) Expression of CD83 on Day 1 of Culture; (E) Expression of CD83 on Day 8 of Culture; (F) Statistical Analysis Results of CD83 on Day 1 and Day 8 of Culture. It is evident that the maturation rate of DCs induced by GM-CSF and IL-4 has been significantly enhanced, thereby facilitating sample preparation for subsequent experiments.Statistical significance is indicated in the figure (*p < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Subsequently, 100 μL of the cell suspension was separately incubated with 5 μL of APC Anti-Human CD1a Antibody and PE Anti-Human CD83 Antibody 5 μL of antibodies (Elabscience Biotechnology Co., Ltd., Wuhan, China).The samples were mixed thoroughly and incubated for 30 min at room temperature in the dark.

Techniques: Expressing, Sample Prep